Application and Modification of the Momose-ohkura Fluorometric Determination of Blood Glucose.

Results of the fluorometric determination of normal and abnormal blood glucose by the method of Momose and Ohkura (2) are lower in many instances than those obtained by reduction methods. The sensitivity of the method was greatly increased by readings at an activation of 470 m, a previously unreported maximum for the reaction product, in 20% sulfuric acid. A modified method is presented which uses a 1 :1000 dilution of 2 ,al. of blood. Protein-free supernate equivalent to 0.5 l. of blood is reacted. Final volume of fluorophore is 10 ml.